上海般诺生物科技有限公司

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Bionoon-64P非接触超声波DNA打断仪 Non-contact ultrasonic DNA interrupter

Bionoon-64P非接触式超声波DNA打断仪,采用等温、非接触的方式,可隔着离心管一次性对64个样品进行打断、匀浆和混合。可用于无菌、超微量破碎以及染色体打断,专为二代测序DNA样本及染色质免疫共沉淀实验样品前处理定制,最少可处理5UL微量样品,适用于珍贵样本。Bionoon-64P Non-contact ultrasonic DNA interrupter uses an isothermal, non-contact method to break, homogenize and mix 64 samples at a time through a centrifuge tube. It can be used for asepsis, ultramicro breakage and chromosome breakage, specially customized for the pre-treatment of second-generation sequencing DNA samples and chromatin immunoprecipitation experiment samples, at least 5UL trace samples, suitable for precious samples.

产品描述

一、产品介绍 Product introduction

Bionoon-64P非接触式超声波DNA打断仪,采用等温、非接触的方式,可隔着离心管一次性对64个样品进行打断、匀浆和混合。可用于无菌、超微量破碎以及染色体打断,专为二代测序DNA样本及染色质免疫共沉淀实验样品前处理定制,最少可处理5UL微量样品,适用于珍贵样本。

Bionoon-64P Non-contact ultrasonic DNA interrupter uses an isothermal, non-contact method to break, homogenize and mix 64 samples at a time through a centrifuge tube. It can be used for asepsis, ultramicro breakage and chromosome breakage, specially customized for the pre-treatment of second-generation sequencing DNA samples and chromatin immunoprecipitation experiment samples, at least 5UL trace samples, suitable for precious samples.

二、产品特点 Product feature

1.高通量:一次性可最多同时处理64个样品,实验效率高。

1. High throughput: up to 64 samples can be processed simultaneously at one time, and the experimental efficiency is high.

2.避免交叉污染:无需频繁操作探头,各种样品在单独的全封闭试管内,避免交叉污染。同时,在密闭的环境下,可有效避免超声过程中产生的气雾或者泡沫扩散到环境中而造成的潜在生物危险。

2. Avoid cross contamination: there is no need to operate the probe frequently, and various samples are in a separate fully enclosed test tube to avoid cross contamination. At the same time, in a closed environment, it can effectively avoid the potential biological hazards caused by the diffusion of aerosol or foam generated during the ultrasonic process into the environment.

3.重复性好、准确性高:超声参数设置灵活,实验步骤标准化,实验重复性好,结果可靠性高。

3. Good repeatability and high accuracy: ultrasonic parameter setting is flexible, experimental steps are standardized, experimental repeatability is good, and the results are highly reliable.

4.样品保护性好:采用4℃低温水浴超声波,能量分布均匀,超声作用完全,在低温环境中,避免样品的变性。

4. Good sample protection: The use of 4℃ low temperature water bath ultrasound, uniform energy distribution, ultrasonic effect is complete, in low temperature environment, to avoid sample denaturation.

5.配置丰富:可配置0.1ml-15ml多种适配器,满足多种样品实验需求;

5. Rich configuration: 0.1ml-15ml variety of adapters can be configured to meet the needs of a variety of samples;

6.实验成本低:无需特殊专用耗材,实验成本相对低。

6. Low experimental cost: no need for special consumables, the experimental cost is relatively low.

三、工作原理  Working principle

通过压电转换器,将电信号转变为高频声波信号,产生数百万的分子“空穴”,利用“空穴”在几秒内变大、破裂产生的瞬时流体剪切力,通过等温、非接触的方式对样本进行打断、匀浆和混合。

Through piezoelectric converters, electrical signals are converted into high-frequency acoustic signals, creating millions of molecular "holes" that utilize "voids.The instantaneous fluid shear force generated by the hole's enlarging and breaking in a few seconds interrupts, homogenizes, and mixes the sample in an isothermal, non-contact manner.

 

四、应用领域   Application fields

■DNA片段化   DNA fragmentation

■RNA 片段化   RNA fragmentation

■ChIP assay(染色质免疫共沉淀)样本前处理

ChIP assay (Chromatin immunoprecipitation) sample pretreatment

■细菌、细胞的破碎及提取膜蛋白

Fragmentation of bacteria and cells and extraction of membrane proteins

■霉菌孢子等的破碎、均质、乳化反应等

Crushing, homogenization, emulsification of mold spores, etc

■贵重试剂的超声处理   Ultrasonic treatment of valuable reagents

■高通量测序仪样本前处理  High-throughput sequencer sample preprocessing

五、技术参数 Technical parameters

1. 采用超声波聚焦原理对样品进行破碎; 

 The sample is broken by ultrasonic focusing principle;

2. 破碎细胞细菌全过程样品均处于完全封闭状态,避免样品交叉污染;

The whole process of broken cell bacteria samples are in a completely closed state to avoid cross-contamination of samples;

3. 超声波DNA打断仪适用于超微量EP管,最低可处理5ul的样品,适用于珍贵样品;

 Ultrasonic DNA interrupter is suitable for ultra-trace EP tube, minimum can handle 5ul samples, suitable for precious samples;

4. 可用于测序DAN样本和CHIP染色体免疫共沉淀实验样本前处理;

 It can be used for pre-processing of sequencing DAN samples and CHIP chromosome immunoprecipitation samples;

5. 核酸样本处理范围100bp-1kb;Nucleic acid sample processing range 100bp-1kb;

6. 样品不接触超声波探头,全程在封闭式管内破碎;

The sample does not touch the ultrasonic probe and is broken in the closed tube throughout;

7. 控制方式:中英文同步触摸屏操作 相位/频率/波形实时显示;

Control mode: real-time display of synchronous touch screen operation phase/frequency/waveform;

8. 控制屏尺寸:≥7寸触摸屏;Control screen size: ≥7 inches touch screen;

9. 频率控制:自动追踪;Frequency control: automatic tracking;

10. 温度系统:温度探头实时探测冰浴,过温自动保护提醒;

Temperature system: temperature probe real-time detection of ice bath, over temperature automatic protection reminder;

11. 处理量:最多64个样品(200ul); Processing capacity: up to 64 samples (200ul);

12. 双语支持:中英文桌面同时显示; Bilingual support: both English and Chinese desktop display;

13. 振幅可调:1-100%;Adjustable amplitude: 1-100%;

14. 数据保存:最多可保存20个数据; Data preservation: up to 20 data can be saved;

15. 振幅调节:0-100%; Amplitude adjustment: 0-100%;

16. 相位/频率: 同时显示;Phase/frequency: display simultaneously;

17.功率可调:1-100%(总功率1000W); Power adjustable: 1-100% (total power 1000W);

18. 制冷系统:外置配套;Refrigeration system: external supporting;

19. 制冷温度:-5℃至100℃;Cooling temperature: -5℃ to 100℃;

20. 时间控制:工作时间0.1s-999min任意可调;间歇时间0.1s-999m任意可调;

Time control: working time 0.1S-999min can be adjusted; Intermittent time 0.1S-999M adjustable;

21. 工作槽尺寸 Working slot size:240x150x100mm;

22. 整机外壳材质:采用开模ABS塑壳一体成型;

The shell material of the whole machine: the ABS plastic shell with open mold is formed;

23. 整机外形尺寸 Overall dimensions (L*W*H):578*360*370mm.

六、配置清单 Configuration list

1、主机1套(含冷却循环系统);

The main machine 1 set (including cooling circulation system);

2. 200ul适配器/单次可最多处理样品64个;

200ul adapter/can handle up to 64samples at a time;

3. 500ul适配器/单次可最多处理样品32个; 

500ul adapter/can handle up to 32samples at a time;

4. 1.5/2ml适配器/单次可最多处理样品20个; 

1.5/2ml adapter/can handle up to 20 samples at a time;

5. 5ml适配器/单次可最多处理样品10个。

5ml adapter/can handle up to 10 samples at a time.